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You are here:Home » Kits and Assays » Kits-ELISA, BioAssay™ » Cryptosporidium (Fecal), Human, BioAssay™ ELISA Kit Not for use in USA, for Export use only

Cryptosporidium (Fecal), Human, BioAssay™ ELISA Kit Not for use in USA, for Export use


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Cryptosporidium (Fecal), Human, BioAssay™ ELISA Kit is an in vitro immunoassay for the qualitative determination of Cryptosporidium antigen in feces. It is a double antibody (sandwich) ELISA using an anti-Cryptosporidium antibody to capture the antigen from the stool supernatant. A second anti-Cryptosporidium antibody is then added which sandwiches the captured antigen. This reaction is visualized by the addition of an anti-second antibody conjugated to peroxidase and the chromogen tetramethylbenzidine (TMB). The resulting blue color development indicates the presence of Cryptosporidium antigens being bound by the anti-Cryptosporidium antibodies.
Catalog #C7944-17
BackgroundCryptosporidium is a coccidian parasite that is recognized as an important enteric pathogen. The organism causes an acute, though self-limiting infection in immunocompetent individuals. Incubation periods of 1 to 12 days have been reported with most oocyst shedding ending by day 21. Symptoms range from mild to severe diarrhea with a variety of complications. The infection in immunocompromised patients is much more severe and may often be life threatening. Passage of fluid, up to 12 liters per day, has been reported. Multiple pathways of Cryptosporidium transmission have been implicated. These include animal to human, water contamination and person-to-person. The latter may include contact between members of the same household, day care centers, and homosexual men. Identification of Cryptosporidium infections was done originally by direct detection techniques. Of these, microscopic examination of stools using stains or fluorescence labeled antibodies has been the most common. However, this method relies on an experienced technician and subsequent observation of intact organisms. Because of the historically low proficiency of correct microscopic examinations, alternative diagnostic methods have been investigated. One important alternative has been the development of an antigen capture ELISA for use with stools. These tests, which have shown comparable sensitivity to experienced microscopic examinations, are fairly simple to perform and do not require the observation of intact organisms.
Kit Components:
1. Microtiter Plate: 1X96wells
2. Reagent 1: 1x11ml
3. Reagent 2 1x11ml
4. Positive Control: 1x2ml
5. Negative Control: 1x2ml
6. Tetramethylbenzidine (TMB): 1x11ml
7. Dilution Buffer: 2x30ml
8. Wash Buffer, 20X: 1x25ml
9. Stop Solution: 1x11ml
Storage and Stability:
Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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