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You are here:Home » Antibodies » Abs to Crystallins » Anti -Crystallin, alpha A

Anti -Crystallin, alpha A

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander’s disease where it accumulates in brain cells of those afflicted.
Catalog #C7944-51
ApplicationsSuitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry (parafin, frozen): 10ug/ml. Staining of alpha-A crystallin in rat thymus samples results in staining of epithelial reticular cells.
Western Blot: 0.1ug/ml. Detects a band of ~20kD representing alpha-A crystallin from bovine lens samples
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceBovine
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenPurified alpha-A crystallin from bovine lens.
SpecificityRecognizes bovine Crystallin, alpha A. Does not detect the unphosphorylated form of the protein. Species Crossreactivity: Rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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