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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IH

Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander’s disease where it accumulates in brain cells of those afflicted.
Catalog #	C7944-51
Applications	Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution	Immunohistochemistry (parafin, frozen): 10ug/ml. Staining of alpha-A crystallin in rat thymus samples results in staining of epithelial reticular cells.
	Western Blot: 0.1ug/ml. Detects a band of ~20kD representing alpha-A crystallin from bovine lens samples
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Bovine
Concentration	~1mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Purified alpha-A crystallin from bovine lens.
Specificity	Recognizes bovine Crystallin, alpha A. Does not detect the unphosphorylated form of the protein. Species Crossreactivity: Rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.