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You are here:Home » Antibodies » Abs to Crystallins » Anti -Crystallin, alpha B (alpha B Crystallin Protein, Alpha(B) Crystallin, Alpha Crystallin B Chain, CRYAB, Crystallin alpha 2, CRYA2, CTPP2, Heat Shock 20 kD Like Protein, Heat Shock Protein beta 5, HSPB5, NY-REN 27 Antigen, Renal Carcinoma Antigen NY-REN-27,

Anti -Crystallin, alpha B (alpha B Crystallin Protein, Alpha(B) Crystallin, Alpha
Crystallin B Chain, CRYAB, Crystallin alpha 2, CRYA2, CTPP2, Heat Shock 20 kD Like
Protein, Heat Shock Protein beta 5, HSPB5, NY-REN 27 Antigen, Renal Carcinoma Antigen
NY-REN-27,

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.
Catalog #C7944-74J
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1:500-1:1000
ELISA: 1ug/ml-3ng/ml
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No10B2612
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2. No preservative added. Azide free
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant protein corresponding to a portion of amino acids within aa1-176 of human CRYAB.
SpecificityRecognizes human alpha B crystallin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesAACRYA, Crystallin alpha Polypeptide 2


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