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You are here:Home » Antibodies » Abs to Crystallins » Anti -Crystallin, alpha B

Anti -Crystallin, alpha B

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit B IP IC
alphalpha-crystallins composed of alphaA (~19kD) and alphaB (~19.2kD) subunits, are major water-soluble proteins accounting for almost 50% of total protein in the mammalian transparent eye lens and they are also found in a variety of other tissues (1). Alpha-crystallins are also referred to as small heat shock proteins, since they are induced by increased temperature in a variety of organisms. (2) The alpha-crystallins have sequence homology as well as structural and functional similarities with the small Hsp’s such as Hsp25/27 (3). Most alpha•crystallins have four common structural and functional features: (i) molecular weight between 12 and 43kDa; (ii) the formation of large oligomeric complexes composed of alphaA-crystallin, alphaB-crystallin and Hsp25/27; (iii) the moderately conserved alpha•crystallin domain in the central region of the protein; and (iv) molecular chaperone activity (2,4). The alpha•crystallin domain comprises approximately 90 residues, is bounded by variable N-terminal and C-terminal extensions and is involved in oligomer assembly. Oligomers can reach 800kDa or more and are dynamic, exhibiting subunit exchanges and organizational plasticity, possibly leading to functional diversity. Phosphorylation of serine residues occurs during development and in response to stress, and usually decreases oligomer size (4). Chaperone activity requires, and is modulated by, oligomerization and is limited to binding unfolded intermediates to prevent irreversible aggregation (2,4). Although productive release and refolding of denatured proteins requires close cooperation with other chaperones. Other proposed functions include a role in membrane stabilization (2) and modulation of intermediate filament organization during physiological stress and neurodegenerative disease (5).
Catalog #C7944-75D
ApplicationsWestern Blot (Colorimetric) (7,8,12): 1:2,000 1:1,000
Western Blot (ECL) (9,11)
Immunocytochemistry: 1:200
Immunoprecipitation (10)
Optimal dilutions to be determined by researcher.PC-Personal Communication
Positive ControlsBovine alphaB-Crystallin Protein
Clone TypePolyclonal
HostRabbit
SourceRat
FormSerum
PuritySerum
ImmunogenA 13 residue synthetic peptide REEKPAVTAAPKK based on the
human alpha-B-crystallin (residues 163-175) (6) and the peptide
conjugated to KLH. This sequence is identical to rat, rabbit and
bovine alpha-B-crystallin over these residues.
SpecificityDetects a ~22kD protein, corresponding to theapparent molecular mass of alpha-B-crystallin on SDS-PAGEimmunoblots, in samples from human, mouse, rat, bovine and porcine. There is no reactivity with beta-crystallin nor alpha-A-crystallin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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