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Anti -Crystallin, alpha B, phosphorylated (Ser45)
Anti -Crystallin, alpha B, phosphorylated (Ser45)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IH IF |
|
| Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are at least 5 dImmunofluorescenceferent proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identImmunofluorescenceied in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander’s disease where it accumulates in brain cells of those afflicted. | | | Catalog # | C7944-75J | | Application(s) | Suitable for use in Immunofluorescence, Immunohistochemistry (parafin embedded) and Western Blot. Other applications not tested. | | Recommended Dilution | Immunofluorescence: 5ug/ml; Immunofluorescence staining of phospho-alpha-B crystallin (Ser45) in U373 MG cells results in cytoplasmic staining. | | Immunohistochemistry (parafin embedded): 8ug/ml | | Western Blot: 0.5ug/ml; Detects a band of ~20kD representing recombinant bovine phospho-alpha-B crystallin (Ser45). | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a lyophilized powder in PBS, 30mg/ml BSA, 0.05% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide: LS pPFYLRPPSF, corresponding to amino acids 44-54 of Human Crystallin (alpha B). | | Specificity | Recognizes Crystallin, alpha B, when phosphorylated at Ser45.Does not detect the unphosphorylated form of the protein. Species Crossreactivity: Bovine, Human, Mouse and Rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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