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You are here:Home » Antibodies » Abs to Crystallins » Anti -Crystallin, alpha B

Anti -Crystallin, alpha B

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Alpha-Crystallins are composed of two gene products; alpha-Crystallin A and B, for acidic and basic, respectively. Alpha-Crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20). They act as molecular chaperones and hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-Crystallin A and B subunits have a 3:1 ratio, respectively. Two additional functions of alpha-crystallins are an autokinase activity and participation in the intracellular architecture. alpha-Crystallin B is expressed widely in many tissues and organs and occurs in many neurological diseases.
Catalog #C7944-75Q
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:2000
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone No7G26
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.01% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant human alpha-Crystallin B (1-175aa) (E. coli)
SpecificityRecognizes human Alpha-Crystallin B
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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