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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -CTBS (Chitobiase, Di-N-acetylchitobiase)

Anti -CTBS (Chitobiase, Di-N-acetylchitobiase)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
CTBS (Di-N-acetylchitobiase; also chitobiase) is a 40kD lysosomal glycoprotein that belongs to glycosyl hydrolase family # 18. It is a reducing-end glycoside hydrolase that serves to degrade asparagines-linked carbohydrates. Mature human CTBS is 347 amino acids (aa) in length and contains one catalytic domain (aa 115-357). There are multiple potential isoforms of CTBS. One shows a three aa deletion of aa 30-32, accompanied by a two two aa substitution for the C-terminal 279 amino acids. There are two potential alternate start sites, one at Met92 and a second at Met207. Mature human CTBS (aa 39-385) is 85% aa identical to mouse CTBS.
Catalog #C7947-28
ApplicationsSuitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1.0ug/ml detection limit for rhCTBS is ~ 0.2ng/well.
Western Blot: 0.1-0.2ug/ml detection limit for rhCTBS is ~ 10ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenNS0-derived rhCTBS. (aa 39 - 385)
SpecificityRecognizes human CTBS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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