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Anti -CTBS (Chitobiase, Di-N-acetylchitobiase)
Anti -CTBS (Chitobiase, Di-N-acetylchitobiase)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B |
|
| CTBS (Di-N-acetylchitobiase; also chitobiase) is a 40kD lysosomal glycoprotein that belongs to glycosyl hydrolase family # 18. It is a reducing-end glycoside hydrolase that serves to degrade asparagines-linked carbohydrates. Mature human CTBS is 347 amino acids (aa) in length and contains one catalytic domain (aa 115-357). There are multiple potential isoforms of CTBS. One shows a three aa deletion of aa 30-32, accompanied by a two two aa substitution for the C-terminal 279 amino acids. There are two potential alternate start sites, one at Met92 and a second at Met207. Mature human CTBS (aa 39-385) is 85% aa identical to mouse CTBS. | | | Catalog # | C7947-28 | | Applications | Suitable for use in Direct ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Direct ELISA: 0.5-1.0ug/ml detection limit for rhCTBS is ~ 0.2ng/well. | | Western Blot: 0.1-0.2ug/ml detection limit for rhCTBS is ~ 10ng/lane under non-reducing and reducing conditions. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | As reported | | Form | Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | NS0-derived rhCTBS. (aa 39 - 385) | | Specificity | Recognizes human CTBS. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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