| Catalog # | C7948-50 |
| Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Supershift Assay. Other applications have not been tested. |
| Recommended Dilution | Western Blot Analysis: 0.1-2.0ug/ml detects CUG-BP1 in HeLa nuclear extract. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with C7948-50 (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. |
| Immunoprecipitation: Immunoprecipitates CUG-BP1 from HeLa cell lysates |
| Immunocytochemistry: Nuclear staining in HeLa and myoblasts from normal patients. |
| Supershift: Supershifts CUG-BP1:DNA complexes in EMSA |
| Optimal dilutions to be determined by the researcher. |
| Recommended secondary antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo |
| I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo |
| Included Positive Antigen Control | H1838: HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 5-10ul of extract and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 1.T.9 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.6, 0.07% sodium azide, before the addition of glycerol to 30% |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Full-length GST fusion protein corresponding to human CUG-BP1, also known as heterogeneous nuclear ribonucleoprotein (hnRNP) hNab50. |
| Specificity | Recognizes human CUG-BP1, Mr 50kD. Species Crossreactivity: Reported to crossreact with mouse, rat, bovine, porcine and rabbit |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
