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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -CUG-Binding Protein 1 Ribonucleoprotein (CUG-BPI, Cytidine Uridine Guanosine Binding Protein 1)

Anti -CUG-Binding Protein 1 Ribonucleoprotein (CUG-BPI, Cytidine Uridine Guanosine Binding
Protein 1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IC
Catalog #C7948-50
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Supershift Assay. Other applications have not been tested.
Recommended DilutionWestern Blot Analysis: 0.1-2.0ug/ml detects CUG-BP1 in HeLa nuclear extract. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with C7948-50 (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: Immunoprecipitates CUG-BP1 from HeLa cell lysates
Immunocytochemistry: Nuclear staining in HeLa and myoblasts from normal patients.
Supershift: Supershifts CUG-BP1:DNA complexes in EMSA
Optimal dilutions to be determined by the researcher.
Recommended secondary antibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo
I1904-19C: IgG, F(abí)2 (HRP) Pab Gt xMo
Included Positive Antigen ControlH1838: HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 5-10ul of extract and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No1.T.9
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.07% sodium azide, before the addition of glycerol to 30%
PurityPurified by Protein G affinity chromatography.
ImmunogenFull-length GST fusion protein corresponding to human CUG-BP1, also known as heterogeneous nuclear ribonucleoprotein (hnRNP) hNab50.
SpecificityRecognizes human CUG-BP1, Mr 50kD. Species Crossreactivity: Reported to crossreact with mouse, rat, bovine, porcine and rabbit
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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