Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IC FC

Cullin proteins assemble a large number of RING E3 ubiquitin ligases and regulate various physiological processes (1). It has been shown that the SKP1, CUL1, F-box (SCF) containing pathway, although similarly used by the mammalian CUL1, is not shared by other cullin members. This implies that most cullins may use a SKP1/F-box-independent pathway to facilitate protein degradation (2). The majority of CUL1 has been found in a complex with CAND1 and ROC1, independent of SKP1 and F-box protein SKP2. Data suggests that CAND1 regulates the formation of the SCF complex, and its dissociation from CUL1 is coupled with the incorporation of F-box proteins into the SCF complex, causing their destabilization (3).
Catalog #	C7949-01E
Applications	Suitable for use inFlow Cytometry, Western Blot, Immunocytochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000-2000
	Immunocytochemistry: 1:100-250
	Flow Cytometry: 1:80
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9E236
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
Purity	Supernatant
Immunogen	A synthetic peptide corresponding to residues in human CUL1.
Specificity	Recognizes human Cullin 1 (CUL-1).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.