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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IP IH IC

Cullin proteins assemble a large number of RING E3 ubiquitin ligases and regulate various physiological processes (1). It has been shown that the SKP1, CUL1, F-box (SCF) containing pathway, although similarly used by the mammalian CUL1, is not shared by other cullin members. This implies that most cullins may use a SKP1/F-box-independent pathway to facilitate protein degradation (2). The majority of CUL1 has been found in a complex with CAND1 and ROC1, independent of SKP1 and F-box protein SKP2. Data suggests that CAND1 regulates the formation of the SCF complex, and its dissociation from CUL1 is coupled with the incorporation of F-box proteins into the SCF complex, causing their destabilization (3).
Catalog #	C7949-01F
Applications	Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions	Western Blot: 1:1000-1:10,000
	Immunohistochemistry: 1:100-1:250
	Immunocytochemistry: 1:100-1:250
	Immunoprecipitation: 1:10
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9E237
Host	Rabbit
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Purity	Supernatant
Immunogen	A synthetic peptide corresponding to residues on the C-terminus of human CUL1.
Specificity	Recognizes human Cullin 1 (CUL-1).
Species Crossreactivity	Mouse and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.