| In yeast, proteolysis of G1 cell cycle regulatory proteins is controlled by a ubiquitin ligase formed by three subunits: Cdc53 (also known as CulA), Skp1 and one of many F-box proteins (reviewed in ref 2). A family of human cullin genes homologous to the S. cerevisiae cdc53 gene has been identi ed (cul1, 2, 3, 4a, 4b, and 5) (1) . It was then shown that Cul1 forms a complex with human Skp1 and the F box protein Skp2. In contrast, Cul2 does not associate with Skp1 and Skp2. Instead it forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongin C, Elongin B and VHL (2) . The meaning of this association is still unknown. Intriguingly, Elongin C shows homology with Skp1 and Elongin B with ubiquitin. In addition, in its active form, SIII contains, instead of VHL, an F box protein: Elongin A. |
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| Catalog # | C7949-13 |
| Applications | Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. |
| Recommended Dilutions | ELISA (Immunogen): 0.1-1.0ug/ml |
| Western Blot: 1-3ug/ml |
| Immunoprecipitation (Native): 5-10ug |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | CUL2 recombinant protein and cell lysates |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~0.25mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide derived from the C-terminus of the human CUL2. |
| Specificity | Recognizes human CUL2 protein at ~83kD. Does not crossreact with the related CUL1 protein. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
