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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum E B IP

In yeast, proteolysis of G1 cell cycle regulatory proteins is controlled by a ubiquitin ligase formed by three subunits: Cdc53 (also known as CulA), Skp1 and one of many F-box proteins. A family of human cullin genes homologous to the S. cerevisiae cdc53 gene has been identi ed (cul1, 2, 3, 4a, 4b, and 5) . It was then shown that Cul1 forms a complex with human Skp1 and the F box protein Skp2. In contrast, Cul2 does not associate with Skp1 and Skp2. Instead it forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongin C, Elongin B and VHL. The meaning of this association is still unknown. Intriguingly, Elongin C shows homology with Skp1 and Elongin B with ubiquitin. In addition, in its active form, SIII contains, instead of VHL, an F box protein: Elongin A.
Catalog #	C7949-13A
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:2,000-1:10,000
	Western Blot: 1:500-1:1,000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid, 0.01% sodium azide.
Purity	Serum
Immunogen	Synthetic peptide, RSQASADEYSYVA, corresponding to aa733-745 of human Cul2, C-terminus (KLH).
Specificity	Recognizes human Cullin 2.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.