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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum E B IP

CUL-3 is a member of the family of human cullin genes (CUL-1,-2,-3,-4a,-4b, and-5), homologous to the S. cerevisiae cdc53 gene. Cullin-3 is proposed to be a part of E3 protein-ubiqutinnn ligase. It forms a tight complex with cyclin E and targets it for ubiquitination, which leads to its degradation by protesomes. Cyclin E is an activator of cdk2, which leads the cell to S phase. Cul-3-/-mice show increased accumulation of Cyclin E protein and disruption of normal cell cycle regulation in vivo. Thus Cullin-3 is important in the regulation of S phase of cell cycle.
Catalog #	C7949-20F
Applications	Suitable for use in ELISA, Western Blot, Immunoprecipitation. Other applications not tested.
Recommended Dilution	ELISA: 1:2000-1:10,000
	Western blot: 1:500-1:1,000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid, 0.01% sodium azide.
Purity	Serum
Immunogen	Synthetic peptide, MSNLSKGTGSRK, corresponding to aa1-12 of human Cul3, N-terminus (KLH).
Specificity	Recognizes human Cullin 3 at ~88.9kD.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.