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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IP FC

Cullin proteins assemble a large number of RING E3 ubiquitin ligases and regulate various physiological processes (1). Cullin 3-based E3 ligase (CUL3) forms a catalytically inactive BTB-CUL3-Rbx1 (BCR) ubiquitin ligase, which becomes functional upon covalent attachment of the ubiquitin Nedd8 (neural precursor cell-expressed, developmentally downregulated 8) near the C terminus of CUL3 (2). CUL3 plays an essential role in both axonal arborization and proper elaboration of dendrites and may require neddylation for its proper function (3). The CUL3 pathway, which selects cyclin E for ubiquitination on the basis of its assembly into CDK complexes, may be one way to control cyclin abundance (4). In a complex with the substrate-specific adaptors KLHL9 and KLHL13, CUL3 is required for correct chromosome alignment in metaphase, proper midzone and midbody formation, and completion of cytokinesis (5).
Catalog #	C7949-21A
Applications	Suitable for use inFlow Cytometry, Western Blot, Immunoprecipitation. Other applications not tested.
Recommended Dilution	Western Blot: 1:20,000-100,000
	Immunoprecipitation: 1:40
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9E238
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
Purity	Supernatant
Immunogen	A synthetic peptide corresponding to residues on the C terminus of human CUL3.
Specificity	Recognizes human Cullin 3.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.