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You are here:Home » Antibodies » Abs to Cullin » Anti -Cullin 4 (CUL-4A)

Anti -Cullin 4 (CUL-4A)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IC
Cullins (1, 2, 3, 4a, 5 and 7) are a family of proteins that assemble a large number of ubiquitin ligases to catalyse polyubiquitiation, as well as binding to various specific factors to recruit substrates. Cullin 4 (CUL 4) serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits of SCF. It plays a role in the polyubiquitination of CDT1 in response to radiation-induced DNA damage and may be important for subsequent DNA repair.
Catalog #C7949-21H1
ApplicationsSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionELISA: 1:500
Western Blot: 1:1000
Immunocytochemistry: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide (MADEAPRKGSFSALVGRTNG) corresponding to aa1-20 of human Cullin 4a
SpecificityRecognizes human CUL 4a
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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