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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

CXCL1, also known as Gro and KC., is an 8kD cytokine which is expressed predominantly by monocytes after activation, but it also expressed by fibroblasts, endothelial cells, synovia cell and several tumor cell lines. CXCL1 promotes neutrophil chemotaxis and degranulation.
Catalog #	C8297-97
Applications	Suitable for use in ELISA, Western Blot and Functional Assays. Other applications not tested.
Recommended Dilutions	ELISA: 0.5-2ug/ml
	Western Blot: 0.1-0.2ug/ml
	Functional Assays: 5-10ug/ml
	Optimal working dilutions to be determined by the researcher.
Recommended Secondary Reagents	I1904-40J: IgG, Fc (HRP) Pab Gt xRb
	Sheep anti Rabbit IgG (HRP)
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O . Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	~1mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.4. No preservatives added. Reconstitute with 100ul sterile ddH2O.
Purity	Purified by affinity chromatography.
Immunogen	Recombinant rat CXCL1.
Specificity	Recognizes rat CXCL1.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.