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You are here:Home » Antibodies » Abs to Growth Factors, Cytokines » Anti -CX3CR1 (Beta Chemokine Receptor-like 1, CCRL1, Chemokine C-X3-C Motif Receptor 1, CX3C Chemokine Receptor 1, C-X3-C CKR-1, CX3C CKR-1, CMK-BRL-1, CMKBLR1, CMKDR1, Fractalkine Receptor, GPR13, GPRV28, V28) (Carboxyfluorescein)

Anti -CX3CR1 (Beta Chemokine Receptor-like 1, CCRL1, Chemokine C-X3-C Motif Receptor 1,
CX3C Chemokine Receptor 1, C-X3-C CKR-1, CX3C CKR-1, CMK-BRL-1, CMKBLR1, CMKDR1,
Fractalkine Receptor, GPR13, GPRV28, V28) (Carboxyfluorescein)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
CX3CR1 is a 40kD seven transmembrane chemokine receptor that is expressed on several T cell subsets, monocytes, macrophages, microglia and epithelial cells. CX3CR1 binding to membrane bound or soluble CX3CR1/Fractalkine promotes inflammatory responses by inducing monocyte adhesion to endothelial cells and macrophage activation. CX3CR1 polymorphisms are associated with the development of chronic inflammatory disorders. Alternately spliced isoforms have extended N-terminal extracellular regions that increase the potency of CX3CR1 as a fusion co-receptor for HIV-1. Human CX3CR1 shares 82% amino acid sequence identity with mouse and rat CX3CR1.
Catalog #C8299-01E
ApplicationsSuitable for Flow Cytometry. Other Appliucacations not tested.
Recommended DilutionsFlow Cytometry: 10ul labels 1x10e6 cells. Tested using human peripherial blood monocytes.
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, add 10ul of C8299-01E to up to 1x10e6 cells and incubate for 30 minutes at RT.
3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (Catalog # F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspend the cells in Flow Cytometry Staining Buffer for flow cytometry analysis. As a control for this analysis, cells in a separate tube should be treated with CFS-labeled mouse IgG1 antibody.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Carboxyfluorescein conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG
Clone No9H106
HostMouse
SourceHuman
Concentration~0.075mg/ml
FormSupplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Carboxyfluorescein.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to the extracellular domain of human CX3CR1 expressed in E. coli.
SpecificityRecognizes human CX3CR1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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