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You are here:Home » Antibodies » Abs to Growth Factors, Cytokines » Anti -CX3CR1 (Beta Chemokine Receptor-like 1, CCRL1, Chemokine C-X3-C Motif Receptor 1, CX3C Chemokine Receptor 1, C-X3-C CKR-1, CX3C CKR-1, CMK-BRL-1, CMKBLR1, CMKDR1, Fractalkine Receptor, GPR13, GPRV28, V28)

Anti -CX3CR1 (Beta Chemokine Receptor-like 1, CCRL1, Chemokine C-X3-C Motif Receptor 1,
CX3C Chemokine Receptor 1, C-X3-C CKR-1, CX3C CKR-1, CMK-BRL-1, CMKBLR1, CMKDR1,
Fractalkine Receptor, GPR13, GPRV28, V28)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B FC
CX3CR1 is a 40kD member of the delta-group of rhodopsin GPCRs. It is expressed on astrocytes, microglia, macrophages, Th1 and Tc1 T cells, NK cells, mouse Gr1lo monocytes plus smooth muscle and mast cells. CX3CR1 mediates adhesion to fractalkine, promotes avid binding of integrins to their ligands, and extends the life of monocytes. Mouse CX3CR1 is a 7-transmembrane protein that is 354aa in length. It contains a 32aa N-terminal extracellular region that shows no glycosylation, and a 56aa C-terminal cytoplasmic domain. Over aa1-32, 91-104, 169-196 and 258-274 collectively, mouse CX3CR1 shares 96% and 77% aa identity with rat and human CX3CR1, respectively.
Catalog #C8299-01G
ApplicationsSuitable for use in Flow Cytometry and Western Blot. Other applications not tested.
Recommended DilutionFlow Cytometry: 2.5ug labels 10e6 cells. Validated with M1 mouse myeloid leukemia cell line.
Western Blot: 1ug/ml detected CX3CR1 in mouse heart tissue and human embryonic heart tissue.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute in 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography
ImmunogenRecombinant protein corresponding to Met1-Thr32, Leu92-Lys104, Thr169-Val196, Lys258-Leu274 from the extracellular domain of mouse CX3CR1 expressed in E. coli (Q9Z0D9).
SpecificityRecognizes mouse CX3CR1. Species Crossreactivity: human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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