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You are here:Home » Antibodies » Abs to Growth Factors, Cytokines » Anti -CXCR4, EL (Fusin, LESTR, HUMSTR, C-X-C Chemokine Receptor 4, CD184)

Anti -CXCR4, EL (Fusin, LESTR, HUMSTR, C-X-C Chemokine Receptor 4, CD184)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified B
Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors(1). Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells(2,3). CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2(4,5). The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1(6,7). CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells(8). CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types(2). Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells(2,5,10). The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites(10,11).
Catalog #C8350-01
ApplicationsSuitable for the detection of CXCR4 by Western blot. Whole cell lysate from HeLa cells can be used as positive control and a 40-45kD band is detected. Western blot analysis of 15 ug of total cell lysate from HeLa cells with anti-CXCR4 (EL) at a concentration of 2ug/ml.
Recommended DilutionWestern blot: 2-4ug/ml
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
PurityPurified IgG fraction
ImmunogenPeptide corresponding to amino acids 182 to 196 in the second extracellular loop (EL) of human CXCR4(3,9).
SpecificityCross reacts with mouse CXCR4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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