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You are here:Home » Antibodies » Abs to G Protein Receptors » Anti -CXCR6 (C-X-C Chemokine Receptor Type 6, CXC-R6, CXCR-6, CDw186, G-protein Coupled Receptor STRL33, G-protein Coupled Receptor Bonzo, CD186, BONZO, STRL33, TYMSTR) (PerCP)

Anti -CXCR6 (C-X-C Chemokine Receptor Type 6, CXC-R6, CXCR-6, CDw186, G-protein Coupled
Receptor STRL33, G-protein Coupled Receptor Bonzo, CD186, BONZO, STRL33, TYMSTR) (PerCP)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified FC
CXCR6, is a G protein- coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.
Catalog #C8375-14B
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 10ul labels10e6 cells. Validated with mouse splenocytes
Optimal dilutions to be determined by the researcher.
Absorption 482nm-564nm
Emission 675nm
Flow Cytometry Validation1. Cells may be Fc-blocked with 1ug of mouse IgG/10e5 cells for 15 minutes at rRT. Do not wash excess blocking IgG from this reaction.
2. After blocking, 10ul of C8375-14B was added to
1x10e6 cells and incubated for 30 minutes at RT
3. Removed unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Mouse Lyse Buffer.
4. Rsuspend the cells in Flow Cytometry Staining Buffer (F4568-10) for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with PerCP-labeled rat IgG2b antibody.
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. PerCP conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No11n54
HostRat
SourceMouse
Concentration~0.05mg/ml
FormSupplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with PerCP.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa1-351 from mouse CXCR6 expressed in CHO cells (Q9EQ16).
SpecificityRecognizes mouse CXCR6.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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