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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

The recombinant CFP (Cyan Fluorescent Protein) is expressed and purified from transformed E. coli using a method that ensures high purity and maximal CFP fluorescence. The protein is suitable as a control reagent for CFP expression studies or as a labeling reagent. Applications include: Use as standards for SDS-PAGE, Western blot analysis of CFP transfected cells, label other proteins, calibration of fluorometers and flow cytometers, fluorescence microscope, or microinjection of CFP into cells and tissues, etc.
Catalog #	C8385-01
Applications	Suitable for use in Western Blot. Other applications have not been tested.
Recommended Dilutions	Western Blot: 0.5-4 ug/ml
	Optimal dilutions to be determined by the researcher.
Recommended Positive Control	C8385-01A: Cyan Fluorescent Protein, Recombinant
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
Purity	Purified by affinitiy chromatography
Immunogen	E. coli expressed Cyan Fluorescent Protein (CFP).
Specificity	Recognizes Cyan Fluorescent Protein (CFP). Crossrectivity: Has a minimum (<5%) crossreactivity with Yellow fluorescent protein.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.