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Specifications

Clone Host Grade Applications Polyclonal Sheep Affinity Purified E B IP IH Gel

Catalog #	C8390
Applications	Suitable for use in ELISA, Immunoprecipitation, Western Blot, Immunohistochemistry, Immunodiffusion and Conjugation. Other applications not tested.
Recommended Dilution	ELISA: 1:5000-1:25,000
	Western Blot: 1:500-1:2500
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Sheep
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, before the addition of glycerol to 40%.
Purity	Purified by immunoaf nity chromatography followed by solid phase adsorption.
Immunogen	A mixture of Cy3 and Cy5 (KLH).
Specificity	Recognizes Cyanine. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum and cyanine conjugated IgG. Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity with other cyanine analogs is unknown.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.