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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Cyanine (Biotin)

Anti -Cyanine (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IH
Catalog #C8390-10
ApplicationsSuitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested.
Recommended DilutionELISA: 1:8000 to 1:40,000 using ABTS as a substrate for 30 min at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, (IgS and protease free), 0.01% sodium azide, 40% glycerol.
PurityPurified by immunoaf nity chromatography.
ImmunogenA mixture of Cy3 and Cy5 conjugated KLH
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-sheep serum and cyanine conjugated IgG. Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analogs is unknown.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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