Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA: 0.5-1ug/mL using 1ug/mL control peptide/well. Western Blot: 1-10ug/mL using ECL Immunohistochemistry: not tested. It is recommended that the antibody be tried at 2-20ug/mL on paraformaldehyde fixed tissue. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
A 20 amino acid peptide sequence near the N-terminus of human CNG1 (1).
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human Cyclic Nucleotide-Gated Channel 1 (CNG1). The immunogen shows no significant sequence homology with other CNGs. Species Crossreactivity: Human. The immunogen sequence is 100% conserved in porcine, 80% in bovine, 70% in canine and 57% in rat.
References
1) Dhallan RS et al (1992) J Neurosci. 12:3248; Distler M et al (1994) Neuropharmacol. 33:1275; Bonigk et al (1993) Neuron 10:865. 2) Ding C et al (1997) Am. J. Physiol. 272:C1335; Pittler SJ et al (1992) J Biol. Chem. 267:6257; Karlson KH et al (1995) BBA 1236:197.
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