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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin A

Anti -Cyclin A

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant E B IP IH
Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk’s). They are expressed temporally to control transition at different specific phases of the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase.
Catalog #C8452-04C
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:100. Incubate for 2hrs at RT
Immunoprecipitation: Native verified
Immunohistochemistry (Formalin-fixed only): 1:40 for 60 min at RT. Staining of formalin/paraffin tissues is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by researcher.
Positive ControlHeLa cells,Tonsil.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No5G86
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid with 0.09% sodium azide.
PurityCulture supernatant
ImmunogenRecombinant N-terminal fragment of human cyclin A protein. Epitope
SpecificityRecognizes human Cyclin A.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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