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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant E B IP IH

Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk’s). They are expressed temporally to control transition at different specific phases of the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase.
Catalog #	C8452-04C
Applications	Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Western Blot: 1:100. Incubate for 2hrs at RT
	Immunoprecipitation: Native verified
	Immunohistochemistry (Formalin-fixed only): 1:40 for 60 min at RT. Staining of formalin/paraffin tissues is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
	Optimal dilutions to be determined by researcher.
Positive Control	HeLa cells,Tonsil.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	5G86
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid with 0.09% sodium azide.
Purity	Culture supernatant
Immunogen	Recombinant N-terminal fragment of human cyclin A protein. Epitope
Specificity	Recognizes human Cyclin A.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.