Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant B IH

Cyclins controls the transitions between the phases of the eukaryotic cell cycle as regulatory subunits of the cyclin dependentkinases (cdk). Human cyclins have been grouped into 5 types, A through E, based largely upon sequence similarity. Cyclin A first identified in sea urchin embryos is a 33kD protein identical to adenovirus e1a-associated protein p60. It can bind and activate the kinases p33cdk2 and p34cdc2. In addition to its mitotic functions, cyclin A is essential for progression through S phase. During S phase, cyclin A forms complex containing cdk2, the pRB-related protein p107, and the transcription factor E2F. It may indeed be directly involved in DNA replication; it colocalizes with the DNA replication complex and was found in a cellular activity that promotes large tumor-antigen-dependent simian virus 40DNA replication in Gl-phase cell extracts. It maps to 4q25-q31 region of human chromosome.
Catalog #	C8452-06B
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:100-1:500
	Immunohistochemistry (formalin fixed paraffin embedded): 1:20-1:40
	Optimal dilutions to be determined by the researcher.
Positive Control	Human lung cell line
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	11E131
Host	Mouse
Source	Human
Concentration	Not determined.
Form	Supplied as a liquid, 0.05% sodium azide.
Purity	Supernatant
Immunogen	Synthetic peptide corresponding to the N-terminal fragment of cyclin A protein. Cellular Localization: Nuclear.
Specificity	Recognizes human Cyclin A.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.