Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin A

Anti -Cyclin A

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IC
Catalog #C8452-08
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml detects p58 Cyclin A in HeLa nuclear extract. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with 2ug/ml C8452-08. Proteins were visualized using goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation 1-5ug/ml. The precipitate may be used to perform a histone H1 kinase assay.
Immunocytochemistry 10ug/ml
Optimal dilution to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O or 40-50% glycerol for long-term storage.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide (KLH-coupled) corresponding to aa 417-432 (C-KHGVSLLNPPETLNL) within the C-terminus of human p58 cyclin A
SpecificityRecognizes human p58 cyclin A. Does not crossreact with cyclin B or cyclin D. Species Crossreactivity: Mouse, rat and sheep
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links