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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin A, CT

Anti -Cyclin A, CT

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's). They are expressed temporally to control transition at different specific phases of the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G-phase.
Catalog #C8452-08K
ApplicationsSuitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 5ug/ml for 2h, room temperature
Immunoprecipitation: 10ug/mg protein lysate
Immunohistochemistry: 1:50 for 30min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.
Positive ControlHela cells, tonsils
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to human cyclin A, at C-terminal.
SpecificityRecognizes human cyclin A. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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