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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin A (BSA & Azide Free)

Anti -Cyclin A (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk’s). They are expressed temporally to control transition at different specific phases of the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase.
Catalog #C8452-17X
MW of Antigen 60kD
Epitope C-terminal
Applications and Recommended DilutionsWestern Blotting (Ab 5-10ug/ml for 2hrs at RT)
Immunoprecipitation (Denatured verified) (Use Protein A) (Ab 10ug/mg protein lysate)
Immunohistochemistry (Formalin/paraffin) ( Ab-6 is better) (Ab 5-10ug/ml for 30 min at RT) ** [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
Positive Control HeLa cells. Tonsil.
Cellular Localization Nuclear
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See C8452-17
PurityPurified by Protein A affinity chromatography.
ImmunogenA synthetic peptide from the C-terminusl of human cyclin A.
SpecificityRecognizes Cyclin A. Species Crossreactivity: human, mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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