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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin B1

Anti -Cyclin B1

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
In mammals, cyclin B associates with inactive p34cdc2 which facilitates phosphorylation of p34cdc2 at Thr-14 and Tyr-15. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates
Thr-14 and Tyr-15 of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B p34cdc2 plays a critical role in G2 to M transition.
Catalog #C8453-03
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not determined.
Recommended DilutionsWestern Blot: 2-4ug/ml for 2hrs at RT
Optimal dilutions to be determined by the researcher.
Positive ControlJurkat cells; Breast carcinoma.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide, before the addition of glycerol to 40%.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide derived from C-terminus of human cyclin B1 protein. Epitope: C-terminus.
SpecificityRecognizes human Cyclin B1. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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