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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

In mammals, cyclin B associates with inactive p34cdc2 which facilitates phosphorylation of p34cdc2 at Thr-14 and Tyr-15. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates
Thr-14 and Tyr-15 of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B p34cdc2 plays a critical role in G2 to M transition.
Catalog #	C8453-03
Applications	Suitable for use in ELISA and Western Blot. Other applications not determined.
Recommended Dilutions	Western Blot: 2-4ug/ml for 2hrs at RT
	Optimal dilutions to be determined by the researcher.
Positive Control	Jurkat cells; Breast carcinoma.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide, before the addition of glycerol to 40%.
Purity	Purified by immunoaffinity chromatography.
Immunogen	A synthetic peptide derived from C-terminus of human cyclin B1 protein. Epitope: C-terminus.
Specificity	Recognizes human Cyclin B1. Species Crossreactivity: mouse and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.