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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP

Catalog #	C8453-06
Applications	Suitable for use in Immunoblot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWarrenImmunoblot	0.5-5ug/ml detecs cyclin B1 in RIPA lysates from human A431 carcinoma cells.
	Immunoprecipitation: 2ug precipitated human cyclin B1 and cdc2 kinase from 500ug of A431 RIPA lysate.
	Optimal dilutions to be determined by the researcher.
Positive Antigen Control	A0001-50
	Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	0.T.183
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Recombinant human cyclin B1.
Specificity	Specific for human cyclin B1 (~ 58kD). Does not crossreact with cyclin A or cyclin D. Species crossreactivity: Human, weak species crossreactivity with mouse was observed..
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.