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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin B1 (CCNB 1, CCNB, CCNB1, G2 mitotic specific cyclin B1)

Anti -Cyclin B1 (CCNB 1, CCNB, CCNB1, G2 mitotic specific cyclin B1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum IH
In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is Ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Catalog #C8453-06D
Cellular Localization Cytoplasmic
Positive Control Breast Carcinoma
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormAs reported
PuritySerum
ImmunogenA synthetic peptide derived from C-terminus of human cyclin B1 protein.
SpecificitySpecies Crossreactivity: The antibody cross reacts with mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCCNB 1, CCNB, CCNB1, G2 mitotic specific cyclin B1


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