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Anti -Cyclin B1 (CCNB 1, CCNB, CCNB1, G2 mitotic specific cyclin B1)
Anti -Cyclin B1 (CCNB 1, CCNB, CCNB1, G2 mitotic specific cyclin B1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
IH |
|
| In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is Ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. | | | Catalog # | C8453-06D | | Cellular Localization | Cytoplasmic | | Positive Control | Breast Carcinoma | | Applications | Suitable for use in Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | As reported | | Purity | Serum | | Immunogen | A synthetic peptide derived from C-terminus of human cyclin B1 protein. | | Specificity | Species Crossreactivity: The antibody cross reacts with mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | CCNB 1, CCNB, CCNB1, G2 mitotic specific cyclin B1 |
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