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Anti -Cyclin B1, CT (G2/Mitotic-specific Cyclin-B1, CCNB1, CCNB)
Anti -Cyclin B1, CT (G2/Mitotic-specific Cyclin-B1, CCNB1, CCNB)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IP IH |
|
| In mammals, cyclin B associates with inactive p34, cdc2 Thr which facilitates phosphorylation of p34 at aa14 and Tyr15. This maintains the inactive state until the end of G2- cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, phase. The inactive cyclin B-p34 dephosphorylates aa 14 Thr Tyr cdc2 and 15 of p34 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin- dependent and is necessary for the cell to exit mitosis. So, cdc2 cyclin B-p34 plays a critical role in G2 to M transition. | | | Catalog # | C8453-07A | | Applications | Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunohistochemistry: 1:200 for 10min at RT | | Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min. | | Optimal dilutions to be determined by the researcher. | | Positive Control | Jurkat cells, breast carcinoma | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to human cyclin B1, at C-terminal. | | Specificity | Recognizes human cyclin B1. Species Crossreactivity: mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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