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Anti -Cyclin B1 (G2/Mitotic-specific Cyclin-B1, CCNB1, CCNB)
Anti -Cyclin B1 (G2/Mitotic-specific Cyclin-B1, CCNB1, CCNB)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
B IP IH FC |
|
| Cyclins were originally discovered as proteins whose accumulation during the interphase and destruction at mitosis played a critical role in the progression through the eukaryotic cell cycle. It was subsequently found that cyclins control the cell cycle by binding to Ser/Thr cyclin-dependent kinases (CDKs) and regulating their activities. Human cyclins have been grouped into 5 types, A through E, based largely upon sequence similarity. Cyclin B is essential for the control of the cell cycle at the G2/M (mitosis) transition. The expression of cyclin B1 is cell-cycle regulated, peaking at the G2/M transition. It interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF), and thus the complex regulates the progression of cells through G2 into M phase. It maps to 5q12 region of human chromosome. | | | Catalog # | C8453-10C | | Applications | Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:50-1:100 | | Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:75 | | Immunohistochemistry (frozen): 1-2ug/ml | | Optimal dilutions to be determined by the researcher. | | Positive Control | Breast carcinoma | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | V152 | | Host | Mouse | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid, 0.05% sodium azide. | | Purity | Purified | | Immunogen | Hamster cyclin B1 protein. Cellular Localization: Cytoplasmic. | | Specificity | Recognizes human Cyclin B1. Species Crossreactivity: mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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