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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin B1

Anti -Cyclin B1

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP FC IF
In mammals, cyclin B associates with inactive p34-cdc2. Cyclin B-p34-cdc2 plays a critical role in G2 to M transition.
Catalog #C8453-15
ApplicationsSuitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended DilutionImmunoprecipitation: 2ug antibody/mg protein lysate. (Use Protein G under native and denatured conditions).
Western Blot: 1-2ug/ml for 2 hours at RT.
Optimal dilutions to be determined by the researcher.
Positive ControlMAD109 or LS174T cells
SourceAscites
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.238
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide at 1mg/ml. See C8453-15X.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman cyclin B1 protein. MW
SpecificityRecognizes Cyclin B1 at 62kD. Species Reactivity: Human, mouse and hamster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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