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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin B1 (BSA & Azide Free)

Anti -Cyclin B1 (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP FC IF
In mammals, cyclin B associates with inactive p34-cdc2. Cyclin B-p34-cdc2 plays a critical role in G2 to M transition.
Catalog #C8453-15X
ApplicationsSuitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended DilutionImmunoprecipitation: 2ug antibody/mg protein lysate
Western Blot: 1-2ug/ml for 2 hours at room temperature
Optimal dilutions to be determined by the researcher.
Positive ControlMAD109 or LS174T cells
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.238
HostMouse
SourceHuman
Concentration1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman cyclin B1 protein.
Cellular LocalizationCytoplasmic
EpitopeAa1-21
MW62kD
SpecificityRecognizes human Cyclin B1 at 62kD. The epitope recognized maps between residues 1-21 of human cyclin B1 protein.
Species crossreactivityMouse and hamster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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