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You are here:Home » Antibodies » Antibodies-Cyclin » Anti -Cyclin B1

Anti -Cyclin B1


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH IF
In mammals, cyclin B associates with inactive p34cdc2. Cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Catalog #C8453-17
MW of Antigen 62kD
Epitope aa1-306
Applications and Recommended DilutionsImmunofluorescence
Immunoprecipitation (Native and denatured) (Use Protein A; Ab 2ug/mg protein lysate)
Immunohistochemistry (Formalin/paraffin) (Use Ab at 2-4ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
Positive Control LS174T cells. Tonsil or breast carcinoma
Cellular Localization Cytoplasmic
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No3F136
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C8453-17X
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman cyclin B1 protein
SpecificityExcellent for staining of formalin/paraffin tissues. Species Crossreactivity: human, mouse, rat hamster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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