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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IP IH IF

In mammals, cyclin B associates with inactive p34cdc2. Cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Catalog #	C8453-17
MW of Antigen	62kD
Epitope	aa1-306
Applications and Recommended Dilutions	Immunofluorescence
	Immunoprecipitation (Native and denatured) (Use Protein A; Ab 2ug/mg protein lysate)
	Immunohistochemistry (Formalin/paraffin) (Use Ab at 2-4ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
Positive Control	LS174T cells. Tonsil or breast carcinoma
Cellular Localization	Cytoplasmic
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	3F136
Host	Mouse
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C8453-17X
Purity	Purified by Protein A affinity chromatography.
Immunogen	Human cyclin B1 protein
Specificity	Excellent for staining of formalin/paraffin tissues. Species Crossreactivity: human, mouse, rat hamster.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.