Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin B1 (BSA & Azide Free)

Anti -Cyclin B1 (BSA & Azide Free)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH IF
In mammals, cyclin B associates with inactive p34cdc2. Cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Catalog #C8453-17X
ApplicationsSuitable for use in Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunoprecipitation (Native and denatured): 2ug/mg protein lysate. Use Protein A.
Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive ControlLS174T cells. Tonsil or breast carcinoma.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3F136
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C8453-17.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman cyclin B1 protein. MW of Antigen
SpecificityRecognizes human Cyclin B1. Species Crossreactivity: mouse, rat and hamster
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links