Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin B1

Anti -Cyclin B1

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IP
In mammals, cyclin B associates with inactive p34cdc2 which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. Ab-2 is excellent for biochemical applications ans co-precipitates cyclin B1 associated p34cdc2.
Catalog #C8453-20
Positive Control Hela or NIH-3T3 cells.
Applications and Suggested DilutionsImmunoprecipitation
(Co-precipitates cdk1/p34cdc2)
Cellular Localization Cytoplasmic
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 15mM sodium azide, 0.2% BSA. Also supplied without BSA and sodium azide. See C8453-20X.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH peptide corresponding to aa 420-433 (Cys-SALVQDLAKAVAKV) from the C-terminal of human cyclin B1.
SpecificityCyclin B1 Ab-2
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links