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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified IP

In mammals, cyclin B associates with inactive p34cdc2 which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. Ab-2 is excellent for biochemical applications ans co-precipitates cyclin B1 associated p34cdc2.
Catalog #	C8453-20
Positive Control	Hela or NIH-3T3 cells.
Applications and Suggested Dilutions	Immunoprecipitation
	(Co-precipitates cdk1/p34cdc2)
Cellular Localization	Cytoplasmic
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	1mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4, 15mM sodium azide, 0.2% BSA. Also supplied without BSA and sodium azide. See C8453-20X.
Purity	Purified by Protein A affinity chromatography.
Immunogen	KLH peptide corresponding to aa 420-433 (Cys-SALVQDLAKAVAKV) from the C-terminal of human cyclin B1.
Specificity	Cyclin B1 Ab-2
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.