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You are here:Home » Antibodies » Antibodies-Cyclin » Anti -Cyclin B1 (BSA & Azide Free)

Anti -Cyclin B1 (BSA & Azide Free)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IP
In mammals, cyclin B associates with inactive p34cdc2 which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. Ab-2 is excellent for biochemical applications ans co-precipitates cyclin B1 associated p34cdc2.
Catalog #C8453-20X
Positive Control Hela or NIH-3T3 cells.
Applications and Suggested DilutionsImmunoprecipitation
(Co-precipitates cdk1/p34cdc2)
Cellular Localization Cytoplasmic
Clone TypePolyclonal
FormSupplied as a liquid in 10mM PBS, pH 7.4. Without BSA and Sodium Azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH peptide corresponding to aa 420-433 (Cys-SALVQDLAKAVAKV) from the C-terminal of human cyclin B1.
SpecificityCyclin B1 Ab-2
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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