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C8453-28D Sheep Anti-CCNB2 (Cyclin B2, G2/Mitotic-specific Cyclin-B2)

Specifications
Clone Type
Polyclonal
Host
Sheep
Source
Human
Swiss Prot
O95067
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Cyclin B2 (also CCNB2 and G2/mitotic-specific cyclin-B2) is a 45-46kD member of the Cyclin AB subfamily, cyclin family of proteins. It is widely expressed and associates with CDK1 providing substrate specificity to a phosphorylating complex. A phosphorCDK1:Cyclin B2 complex is associated with the Golgi apparatus, where it contributes to Golgi fragmentation during mitosis. It is also possible that Cyclin B2 can substitute for Cyclin B1 during the early stages of mitosis. Human Cyclin B2 is 398aa in length. It contains two cyclin box folds (aa201-290 and 298-383) and two substrate binding sites (aa165-254 and 264-347). Phosphorylation occurs at Ser92, Thr94, Ser99, Ser204, Ser392 and Ser398. Over aa1-101, human Cyclin B2 shares 79% aa identity with mouse Cyclin B2.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot:1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Purified, E. coli-derived recombinant human Cyclin B2 (aa1-101; Accession #O95067).
Form
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Cyclin B2. Species sequence homology: mouse (79%).
Conjugates
Pricing
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