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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin C

Anti -Cyclin C

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IF
Cyclin C was originally identified by a genetic screen for human and Drosophila cDNAs that complement a triple knock-out of the CLN genes in Saccharomyces cerevisiae. Cyclin C is a nuclear protein present in a multiprotein complex. It interacts both in vitro and in vivo with cdk8, a novel protein-kinase of the cdk family. It is suggested that cyclin C and cdk8 control RNA polymerase II function. Human cdk8-cyclin C might be functionally associated with the mammalian transcription apparatus, perhaps involved in relaying growth-regulatory signals. Ab-1 is superb for biochemical applications including co-precipitation of cdk8.
Catalog #C8453-30
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunofluorescence
Immunoprecipitation (Co-precipitates cdk8)
Western Blot: 5ug/ml for 2hrs at RT
Optimal dilutions to be determined by the researcher.
Cellular Localization Nuclear
Positive Control HeLa or NIH-3T3 cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 15mM sodium azide, 0.2% BSA. Also supplied without BSA and sodium azide. See C8453-30X.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa 290-303 (Cys-QGPNGSQNSSYSQS) from the C-terminal of human cyclin C.
SpecificityRecognizes human Cyclin C Species Crossreactivity: canine, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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