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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum E B IH

Cyclin E is a regulatory subunit of cdk-2 and controls G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues. Qualitative and quantitative alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
Catalog #	C8453-31
Applications	Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000-1:5000
	ELISA: 1:50,000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	Not determined.
Form	Supplied as a lyophilized powder in PBS, pH 7.2, 0.1% sodium azide before the addition of glycerol to 40%. No stabilizing proteins added.
Purity	Serum.
Immunogen	Synthetic peptide corresponding to the C terminal of human Cyclin E (KLH).
Specificity	Recognizes human Cyclin E at 45kD. IEP Assay shows a single precipitin arc against source serum protein and IgG. No reaction was observed against other related cyclins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.