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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IP IH IC FC

Cyclin B1 regulates mitosis in conjunction with Cdc2 kinase to form the Cdc2-cyclin B1 complex also known as maturation promoting factor (MPF) (1-2). The activation of the complex begins when the regulatory subunit, cyclin B1, associates with the catalytic subunit of Cdc2. Cyclin B1 levels rise during S phase and G2, and peak at mitosis. The timing of mitosis can also be influenced by changes in cyclin B1–Cdc2 localization. Cyclin B1 is mostly located in the cytoplasm during S and G2 phases and then rapidly accumulates in the nucleus at the onset of prophase (3).
Catalog #	C8453A
Applications	Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution	Flow Cytometry: 1:100
	Western Blotting: 1:5,000-20,000
	Immunohistochemistry: 1:100
	Immunocytochemistry: 1:50
	Immunoprecipitation: 1:100
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	6k138
Host	Rabbit
Source	Human
Form	Supplied as a liquid
Purity	Supernatant
Immunogen	A synthetic peptide corresponding to residues in C-terminus of human cyclin B1
Specificity	Recognizes human cyclin B1
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.