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You are here:Home » Antibodies » Antibodies-Cyclin » Anti -Cyclin D1

Anti -Cyclin D1


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH FC
Cyclin D1 is a nuclear protein (36kD ). D type cyclins are predominantly expressed in the G1 phase of the cell cycle. Cyclin D1 study is of interest in various forms of malignancy and over expression has been linked to poor prognosis.
Catalog #C8454-01B
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry (frozen and paraffin), ELISA, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:2000
Flow Cytometry: Neat; Use 5ul of the optimal working dilution to stain 10e6 cells in 100ul. Permeabilization is recommended prior to staining.
Immunohistochemistry (Paraffin and frozen): 1:1000-1:10,000 Note: Paraffin sections require antigen retrieval using sodium citrate buffer, pH 6.0, heat treatment prior to staining.
Optimal working dilutions to be determined by researcher.
Recommended Positive Control TissueSmall intestine, colon.
Recommended Secondary ReagentsFlow Cytometry:
I1904-66A: IgG, F(abí)2, X-Adsorbed (FITC) Pab Rb xMo
I1903-08L: IgG, F(abí)2 (Rhodamine) Pab Rb xMo
I1903-08M: IgG, F(abí)2, X-Adsorbed (Rhodamine) Pab Rb xMo
Immunohistochemistry (Paraffin and frozen):
I1904-65W: IgG, F(abí)2 (HRP) Pab Rb xMo
I1904-66C: IgG, F(abí)2, X-Adsorbed (HRP) Pab Rb xMo
Recommended Negative ControlHuman Samples: I1904-78R: IgG1 Negative Control
Rat Samples: I1904-78Y: IgG1 Negative Control Mab Mo xHu
HybridomaSpleen cells from immunized mice were fused with cells of the mouse Sp-2 myeloma cell line.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No3H2043
FormSupplied as a liquid in Tris-glycine, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman Cyclin D1
SpecificityRecognizes the Cyclin D1 (36kD). Species Crossreactivity: Human and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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