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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin D1

Anti -Cyclin D1


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC N
Catalog #C8454-01D
Cyclin D1 gene is identical to the BCL-1 and the PRAD-1 oncogene and is part of the chromosome 11q13 amplicon which is amplified in several common tumor types like breast, lung, esophagus, urinary bladder and head and neck carcinoma. This antibody is especially useful in tumor diagnosis it can be used as fast method to identify cases with 11q13 amplifications. Polypeptide reacting: Mr 36,000 polypeptide (cyclin D1 polypeptide of human cells).
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry and Neutralization. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Frozen and paraffin): 1:10 with PBS, pH 7.4; Incubation Time 1 hr at RT or overnight at 4°C for paraffin sections. Paraffin without microwave treatment only after fixation in methacarn, ethanol or phenol formalin; weak reaction using routine formol-fixed paraffin sections. Paraffin with microwave treatment for routine formalin-fixation.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No5F184
Concentration~0.05mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman recombinant full-length cyclin D1 protein.
SpecificityRecognizes human cyclin D1 protein present predominantly in G1 phase of cell cycle. It does not crossreact with D2 or D3 cyclins (the closest relatives within the cyclin family). Species Crossreactivity: monkey, rat, mouse, canine and rodent.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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