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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant E B IH

Cyclin D1 or PRAD-1 or bcl-1 is one of the key cell cycle regulators, and functions in association with cdk4 and / or cdk6 by phosphorylating the Rb protein. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL).
Catalog #	C8454-01G
Applications	Western Blot and Immunohistochemistry (Formalin/paraffin). Other applications have not been tested.
Recommended Dilutions	Immunohistochemistry: 1:100 for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions to be determined by researcher.
Positive Control	Breast carcinomas or mantle cell lymphoma.
Cellular Localization	Nuclear.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	5G87
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid with 0.09% sodium azide.
Purity	Culture supernatant
Immunogen	A synthetic peptide from C-terminus of human cyclin D1.
Specificity	Recognizes Cyclin D1. Species Crossreactivity: Human, Mouse and Rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.