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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin D1 (PRAD-1, bcl 1) (FITC)

Anti -Cyclin D1 (PRAD-1, bcl 1) (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC IF
Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk), which control transition at different specific phases of the cell cycle. In mammalian cells, Cyclin D1 plays a critical role in G1 to S transition. Cyclin D1 is a putative proto-oncogene.
Catalog #C8454-02B
ApplicationsSuitable for use in Immunofluorescence and Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.5-1.0ug to stain 106 cells fixed in 75% ethanol.
Optimal dilutions to be determined by the researcher.
Positive ControlUnstimulated human PBMCs, T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, U-2-OS, HT29, Ramos, Jurkat, KNRK, C32, A431 cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No5J223
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 15 mM sodium azide. Labeled with fluorescein isothiocyanate (FITC).
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman recombinant full length Cyclin D1 protein
SpecificityRecognizes human Cyclin D1 at ~36kD. Species Crossreactivity: Human, monkey, mouse, rat, and canine Cyclin D1. Other species were not tested.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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