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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH FC IF

Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk), which control transition at different specific phases of the cell cycle. In mammalian cells, Cyclin D1 plays a critical role in G1 to S transition. Cyclin D1 is a putative proto-oncogene.
Catalog #	C8454-02B
Applications	Suitable for use in Immunofluorescence and Flow Cytometry. Other applications not tested.
Recommended Dilution	Flow Cytometry: 0.5-1.0ug to stain 106 cells fixed in 75% ethanol.
	Optimal dilutions to be determined by the researcher.
Positive Control	Unstimulated human PBMCs, T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, U-2-OS, HT29, Ramos, Jurkat, KNRK, C32, A431 cells.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	5J223
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 15 mM sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity	Purified by Protein A affinity chromatography.
Immunogen	Human recombinant full length Cyclin D1 protein
Specificity	Recognizes human Cyclin D1 at ~36kD. Species Crossreactivity: Human, monkey, mouse, rat, and canine Cyclin D1. Other species were not tested.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.