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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin D1 (PRAD-1, bcl 1)

Anti -Cyclin D1 (PRAD-1, bcl 1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH
Cyclin D1 is a 44kD cell cycle regulatory protein whose expression level and nuclear/cytoplasmic distribution are tightly regulated in synchrony with the cell cycle. Cyclin D1 complexes containing Cdk4 or Cdk6 induce phosphorylation of Rb, a requirement for progression through the G1/S cell cycle transition. Human Cyclin D1 shares 93% amino acid sequence identity with mouse and rat Cyclin D1.
Catalog #C8454-02D
ApplicationsSuitable for use in Immunohistochemistry and ELISA. Other applications not tested.
Recommended DilutionImmunohistochemistry: 10ug/ml
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No7H91
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenE. coli-derived recombinant human Cyclin D1 (aa1-295) Accession # NP_444284
SpecificityRecognizes human Cyclin D1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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