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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IH

Cyclin D1 is a 44kD cell cycle regulatory protein whose expression level and nuclear/cytoplasmic distribution are tightly regulated in synchrony with the cell cycle. Cyclin D1 complexes containing Cdk4 or Cdk6 induce phosphorylation of Rb, a requirement for progression through the G1/S cell cycle transition. Human Cyclin D1 shares 93% amino acid sequence identity with mouse and rat Cyclin D1.
Catalog #	C8454-02D
Applications	Suitable for use in Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilution	Immunohistochemistry: 10ug/ml
	ELISA: 0.5-1ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	7H91
Host	Mouse
Source	Human
Concentration	As reported
Form	Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
Purity	Purified by Protein G affinity chromatography.
Immunogen	E. coli-derived recombinant human Cyclin D1 (aa1-295) Accession # NP_444284
Specificity	Recognizes human Cyclin D1.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.