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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin D1

Anti -Cyclin D1

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
Cyclin D1 belongs to the Cyclin D family. Cyclin D1 is required for the cell cycle G1/S transition (1). Cyclin D1 forms a serine/threonine kinase holoenzyme complex with and functions as a regulatory subunit of CDK4 or CDK6. Cyclin D1 also phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1/S phase (2-3). Amplification or overexpression of cyclin D1 plays a pivotal role in the development of various human cancers including parathyroid adenoma, breast cancer, colon cancer, lymphoma, melanoma, and prostate cancer (4). Over-expression of cyclin D1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis (5). Cyclin D1 also associates with, and regulates the activity of transcription factors, coactivators and corepressors that govern histone acetylation and chromatin remodeling proteins (6-7)
Catalog #C8454-02G
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:10,000-50,000
Immunohistochemistry: 1:100-250
Immunocytochemistry: 1:100-250
Immunoprecipitation: 1:40
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E240
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human Cyclin D1.
SpecificityRecognizes human Cyclin D1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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